Our Services
We spent decades in industry settings refining the services we provide and understand the importance of working with experienced teams. As a result, we focus our efforts on a select group of services to deliver the highest quality data and reagents with unparalleled expertise. Our core services are divided into three areas and encompass:
DEL-readiness and optimization
Custom protein production and analysis
High-throughput assay development and ligand binding studies
DEL-Readiness and Optimization
QC and Optimization for DEL
DNA-encoded library (DEL) technology is becoming a standard method of small molecule discovery across the industry. Success of DEL campaigns are reliant on production and characterization of the proteins used for screening. Our experience with DEL screening allows us to provide a package of key optimization metrics, including:
• Construct design/Protein production
• Protein characterization (e.g., activity, monodispersity and stability)
• DEL specific buffer optimization
• On bead protein characterization
• Channel selection-specific conditions
Piloting DEL
Several leading DEL-providers now offer do-it-yourself DEL kits for piloting and small-scale DEL screens. Our experience working with these kits can help avoid the common mistakes and increase the chance of successful DEL hit-finding.
Custom Protein Production and Analysis
Expression and Purification of Proteins and Protein Complexes
We offer protein production in both bacterial and baculovirus-insect cell systems with multiple services including:
• Vector production and QC
• Test expression and pilot purification
• Scale up protein production and QC (SDS-PAGE, SEC, Activity)
• Multiple Tag systems available with the option for removal after purification
• QC summaries and batch records for production and purification
Rapid Purification and Analysis
Our rapid purification and QC protocols allow us to deliver high-quality proteins and protein complexes with:
• High specific activity
• Low background contaminants
• Native PTMs from insect cells
• Less degradation compared to longer purification schemes
High-Throughput Assay Development and Ligand Binding Studies
Low and High-Througput Assay Development
Our industry-formed assay development experience allows us to offer a range of enzymatic assays and novel assay development for a range of enzyme classes including:
• Colorimetric and fluoroescnce
• End-point and kinetic
• Plate-based (e.g., 96 and 384-well)
• Non-plate based (e.g., gel and filter binding)
Ligand Binding Studies
Our drug discovery experience enables the timely development of assays for both protein characterization and lead finding/characterization efforts. We can supply assays developed on several technology platforms such as:
• DSF
• Fluorescence Polarization (FP)
• TR-FRET